منابع مشابه
The glycosaminoglycans of human plasma.
A method is proposed for the measurement of glycosaminoglycans (GAG) on 5-10 ml of plasma. It is based on the adsorption of GAG on small ECTEOLA columns followed by measurement of the hexuronic acid in the NaCl eluates. Routine use of the method has indicated the presence of a GAG fraction that adsorbs readily on ECTEOLA ("free" GAG) and of another that adsorbs on it only after treatment with p...
متن کاملThe Interaction of Human Plasma Glycosaminoglycans with Plasma Lipoproteins
Formalinized, tannic acid-treated sheep erythrocytes coated with low density lipoproteins (LDL) or apoprotein B (apo-B) are agglutinated by anti-apo-B immunserum. Those coated with high density lipoproteins (HDL) or apoprotein A-I (apo-A-I) are agglutinated by anti-apo-A-I immunserum. These coated formocells have been used to study the interactions of lipoproteins and apoproteins with plasma gl...
متن کاملThe interaction between human plasma lipoproteins and connective tissue glycosaminoglycans.
The interaction between human plasma lipoproteins and glycosaminoglycans has been studied by use of gels consisting of cross-linked hyaluronic acid and of sulfated glycosaminoglycans covalently attached to agarose (Sepharose 4B). When chromatographed on a column of granulated hyaluronic acid gel at physiological pH and ionic strength, very low density (VLDL), low density (LDL), and high density...
متن کاملIsolation and characterization of glycosaminoglycans in human plasma.
We have described methodology for the isolation and quantitation of glycosaminoglycans present in human plasma. Plasma glycosaminoglycans can be quantitatively adsorbed on a DEAE-Sephacel ion exchanger and eluted with a salt gradient as two groups: a low-charge fraction and a high-charge fraction. The low-charge fraction consists of chondroitin sulfate with a low sulfate content and the high-ch...
متن کاملGlycosaminoglycans affect the interaction of human plasma kallikrein with plasminogen, factor XII and inhibitors.
Human plasma kallikrein, a serine proteinase, plays a key role in intrinsic blood clotting, in the kallikrein-kinin system, and in fibrinolysis. The proteolytic enzymes involved in these processes are usually controlled by specific inhibitors and may be influenced by several factors including glycosaminoglycans, as recently demonstrated by our group. The aim of the present study was to investig...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1969
ISSN: 0021-9738
DOI: 10.1172/jci105989